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X-gal staining was performed for detecting β-galactocidase reporter expression in a wide variety of adult tissues of each trap line.
2.7% formalin
0.2% glutaraldehyde
0.02% Nonidet P-40
in PBS
2% X-gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) in
dimethylformamide
Store at -20℃ in small aliquots.
5mM Potassium ferricyanide
5mM Potassium ferrocyanide
0.5mM MgCl2
0.05% X-gal stock solution
in PBS
Make fresh just before use.
Note: Tissues were rocked gently at room temperature on each step.