Method 3'-Plasmid Rescue

Method	3'-Plasmid Rescue
Description	It is necessary to determine the genomic sequence which is adjacent to the inserted trap vector in order to distinguish the homozygote from the heterozygote. One of the methods to clone the genomic fragment that is adjacent to the vector is a plasmid rescue. This method utilizes the plasmid DNA with replication origin in the trap vector. It contains three steps; (i) digest with a suitable restriction enzyme (RE), (ii) self-ligation, and (iii) transformation of the E. coli.