Vector | pU-21B |
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Description | pU-21B (Accession number: AB255647, 9399 bp) is a “Promoter Trap” vector with three in-frame stop codons with the ATG of the beta-geo. Integration site is concentrated in the 5’-end of a trapped gene. EN2-intron; 1.2 kb of Mouse En2 intron 1. EN2-SA; splice acceptor of mouse En2 exon 2. beta-geo; fusion of beta-galactosidase and neomycin phosphotransferase. pA1; SV40 polyadenylation signal. pA2; mouse PGK gene polyadenylation signal. |