ID K13F05

Registered: 2011.04.03   Last update: 2016.01.06
Gene Name PHD finger protein 20 Gene Symbol Phf20
Chromosome 2 Genomic Location chr2:156,020,000-156,140,000
Synonyms 6820402O20Rik
Links UCSC Genome Browser(chr2:156,020,000-156,140,000)
NCBI Gene(228829)
IGTC(Phf20,870)
UNIGene(Mm.427078)
 MGI(2444148)
KEGG GENES(mmu:228829)
EST Profile(mm.427078)
Other Clone Trapped This Gene
21-42, 21-T224, 21-W269, 21-KBW263
Trap Vector pCMT-SAhygpA-NP21 Cell Line vdR2-4 Method Splinkerette PCR
Accession AB623065 GSS Location chr2:156,024,980-156,025,268 Size 289
Sequence TTACAGACTTGAGGACTGCCTGGACTACAGGATGAGTTCAAGACTAGCTAGGGATGCATCTCAAA
CAATCTGCTTAGTTGGTTCTAAGGCTCTTAGGTTCTGCTCCTCCCCGCACATCCTCCTCCCTCTC
CTTTTCCTTTTTCCTTTTTTACATTTTTATTTTTTGAGTTAGGATTTCTCTATGTAGCCTGACAC
TCCGGCTTTCCTGGAACTAGCNCTGTAAATTAGGCTGACCTCAAACTCGACAGAGATTCGGCTTT
CTCTGCCTTCTTAGTGCTGATATTAAAGG
Links UCSC Browser(chr2:156,024,980-156,025,268)
IGTC(AyuK13F05)

Homology Search Results

Mouse Information

Card ID
Strain Name
Internal Code
Description This clone was isolated by using the gene trap vector pCMT-SAhygpA-NP21 and the ES cell line vdR2-4. Homozygous mutation was introduced at the vector insertion site by exploiting mitotic recombination induced with transient disruption of the Bloom’s syndrome gene. This double knock out ES cell line was established by Takeda Laboratory at Osaka University, and deposited to the EGTC. Please check Homozygous ES Cell Database. This sequence-tag is genomic sequence of 3'-flanking region obtained by Splinkerette PCR.
[Paper] "A homozygous mutant embryonic stem cell bank applicable for phenotype-driven genetic screening." Horie, K., Kokubu, C., Toshida, J., Akagi, K., Isotani, A., Oshitani, A., Yusa, K., Ikeda, R., Huang, Y., Bradley, A. and Takeda, J., Nature Methods, 8, 1071-1077 (2011). PubMed ID:22020066.
Links IMSR (for Phf20)